Bioorthogonally applicable multicolor fluorogenic naphthalimide–tetrazine probes with aggregation-induced emission characters
Yongcheng Wang, Yu Teng, Hong Yang, Xiang Li, Dali Yin, Yulin Tian
Abstract
tetrazines were developed as bioorthogonal fluorogenic probes, which could produce significant fluorescence enhancement, notable aggregation-induced emission (AIE) characters and multicolor emissions after bioorthogonal reaction with strained dienophiles. Manipulating the π-bridge in the fluorophore skeleton allows fine-tuning of the emission wavelength and influences the AIE-active properties. With these probes, we succeeded in no-wash fluorogenic protein labeling and mitochondria-selective bioorthogonal imaging in live cells.
Topics & Concepts
Bioorthogonal chemistryTetrazineFluorophoreAggregation-induced emissionFluorescenceChemistryCombinatorial chemistryNanotechnologyClick chemistryMaterials scienceOrganic chemistryPhysicsQuantum mechanicsClick Chemistry and ApplicationsLuminescence and Fluorescent MaterialsAdvanced biosensing and bioanalysis techniques