Efficient Suppression of Natural Plasmid-Borne Gene Expression in Carbapenem-Resistant Klebsiella pneumoniae Using a Compact CRISPR Interference System
Shigang Yao, Dawei Wei, Na Tang, Yuqin Song, Chao Wang, Jie Feng, Gang Zhang
Abstract
There is an urgent need for efficient tools for genetic manipulation to assess plasmid function in clinical drug-resistant bacterial strains. To address this need, we developed an all-in-one CRISPR interference (CRISPRi) system that easily inhibited the gene expression of a natural multidrug-resistant plasmid in an sequence type 23 (ST23) Klebsiella pneumoniae isolate.
Topics & Concepts
Klebsiella pneumoniaePlasmidCRISPRBiologyGeneKlebsiellaMicrobiologyEscherichia coliGeneticsAntibiotic Resistance in BacteriaCRISPR and Genetic EngineeringVibrio bacteria research studies