Detection and Stability of SARS-CoV-2 Fragments in Wastewater: Impact of Storage Temperature
Rudolf Markt, Markus Mayr, Evelyn Peer, Andreas Otto Wagner, Nina Lackner, Heribert Insam
Abstract
SARS-CoV-2 wastewater epidemiology suffers from uncertainties concerning sample storage. We show the effect of the storage of wastewater on the detectable SARS-CoV-2 load. Storage at 4 °C for up to 9 days had no significant effect, while storage at -20 °C led to a significant reduction in gene copy numbers.
Topics & Concepts
WastewaterSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Environmental scienceCoronavirus disease 2019 (COVID-19)2019-20 coronavirus outbreakWaste managementVirologyBiologyEnvironmental engineeringEngineeringMedicineDiseasePathologyInfectious disease (medical specialty)OutbreakSARS-CoV-2 detection and testingBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniques