AAV delivery strategy with mechanical support for safe and efficacious cardiac gene transfer in swine
Renata Mazurek, Serena Tharakan, Spyros A. Mavropoulos, Deanndria T. Singleton, Olympia Bikou, Tomoki Sakata, Taro Kariya, Kelly P. Yamada, Erik Kohlbrenner, Lifan Liang, Anjali J. Ravichandran, Shin Watanabe, Roger J. Hajjar, Kiyotake Ishikawa
Abstract
Adeno-associated virus-based gene therapy is a promising avenue in heart failure treatment, but has shown limited cardiac virus uptake in humans, requiring new approaches for clinical translation. Using a Yorkshire swine ischemic heart failure model, we demonstrate significant improvement in gene uptake with temporary coronary occlusions assisted by mechanical circulatory support. We first show that mechanical support during coronary artery occlusions prevents hemodynamic deterioration (n = 5 female). Subsequent experiments show that coronary artery occlusions during gene delivery improve gene transduction, while adding coronary sinus occlusion (Stop-flow) further improves gene expression up to >1 million-fold relative to conventional intracoronary infusion. Complete survival during and after delivery (n = 10 female, n = 10 male) further indicates safety of the approach. Improved cardiac gene expression correlates with virus uptake without an increase in extra-cardiac expression. Stop-flow delivery of virus-sized gold nanoparticles exhibits enhanced endothelial adherence and uptake, suggesting a mechanism independent of virus biology. Together, utilizing mechanical support for cardiac gene delivery offers a clinically-applicable strategy for heart failure-targeted therapies.