Easy-Prime: a machine learning–based prime editor design tool
Yichao Li, Jingjing Chen, Shengdar Q. Tsai, Yong Cheng
Abstract
Prime editing is a revolutionary genome-editing technology that can make a wide range of precise edits in DNA. However, designing highly efficient prime editors (PEs) remains challenging. We develop Easy-Prime, a machine learning-based program trained with multiple published data sources. Easy-Prime captures both known and novel features, such as RNA folding structure, and optimizes feature combinations to improve editing efficiency. We provide optimized PE design for installation of 89.5% of 152,351 GWAS variants. Easy-Prime is available both as a command line tool and an interactive PE design server at: http://easy-prime.cc/ .
Topics & Concepts
Prime (order theory)BiologyHuman geneticsGenome BiologyMachine learningComputational biologyArtificial intelligenceComputer scienceGeneticsGenomicsCombinatoricsMathematicsGenomeGeneEvolutionary Algorithms and ApplicationsCRISPR and Genetic EngineeringProtein Degradation and Inhibitors