Quality by design approach for green HPLC method development for simultaneous analysis of two thalassemia drugs in biological fluid with pharmacokinetic study
Michel Y. Fares, Maha A. Hegazy, Ghada M. El‐Sayed, Maha M. Abdelrahman, Nada S. Abdelwahab
Abstract
ibuprofen (IBF) as an internal standard. The established method's greenness profile was evaluated and measured using various assessment tools, and the developed method was green. For the validation of the developed method, FDA recommendations were followed, and all the results obtained met the acceptance criteria. The suggested method was successfully used to study the pharmacokinetic parameters of DFX and DFP in rat plasma. Due to the substantial increase in bioavailability of the two iron chelating drugs, the results from this study strongly recommend their co-administration.
Topics & Concepts
DeferipronePharmacokineticsDeferasiroxThalassemiaHigh-performance liquid chromatographyQuality by DesignChromatographyChemistryBiochemical engineeringComputer sciencePharmacologyMedicineEngineeringInternal medicinePhysical chemistryParticle sizeAnalytical Methods in PharmaceuticalsAntibiotics Pharmacokinetics and EfficacyPesticide Residue Analysis and Safety