Effect of pesticides on erythrocytes of indigenous fish Labeo rohita
Naheed Bano, Amir Nadeem, Sadia Maalik, Sajida Mushtaq, Naeem Iqbal, Ahmad Khan, Asghar Shabbir, Muhammad Imran, Safia Niaz, Muhammad Yasin, Mirza Abdul Qayyum, Bashir Ahmad, Milan Skalický, Sulaiman Ali Alharbi, Saleh Alfarraj
Abstract
Single cell gel electrophoresis was used to observe the effect of bifenthrin and chlorpyrifos on Labeo rohita. The present work was planned to study the genotoxic effect of pesticide mixture on the major indian carp, L. rohita at sub-lethal concentration 33% LC50. First of all, 96-hr LC50 was determined for L. rohita, then fish was given sub-lethal environment for 70 days. Erythrocytes from blood were taken after each fortnight for the assessment of time dependent DNA damage (% damage), aggregate tail length of comets and genomic destruction. There was increase in damage up to 56 days and after that a slight decrease was observed in next fourteen days. After statistical analysis, it was concluded that the Single Cell Gel Electrophoresis may be implemented in ecofriendly observer courses in evaluation of toxicity.