Needle in a Whey-Stack: PhRACS as a Discovery Tool for Unknown Phage-Host Combinations
Eoghan Casey, Brian McDonnell, Kelsey White, Panagiota Stamou, Tadhg Crowley, Ian O’Neill, Katherine Lavelle, Stephen J. Hayes, Gabriele Andrea Lugli, Silvia Arboleya, Kieran James, Marco Ventura, Inés Martínez, Miguel Gueimonde, Fabio Dal Bello, Kenneth Nally, Jennifer Mahony, Douwe van Sinderen
Abstract
genetic analyses (i.e., phageomic studies) and traditional culture-based methodology. Through the labeling of specific bacterial hosts with fluorescently tagged recombinant phage receptor binding proteins and the isolation of tagged cells using flow cytometry, PhRACS allows the full potential of phageomic data to be realized in the wet laboratory.
Topics & Concepts
Phage displayIn silicoFlow cytometryComputational biologyRecombinant DNAHost (biology)Isolation (microbiology)BiologyChemistryMicrobiologyMolecular biologyGeneticsAntibodyGeneBacteriophages and microbial interactionsMonoclonal and Polyclonal Antibodies ResearchBacterial Genetics and Biotechnology