Myogenic Progenitor Cell Lineage Specification by CRISPR/Cas9-Based Transcriptional Activators
Jennifer B. Kwon, Ashish Vankara, Adarsh R. Ettyreddy, Joel D. Bohning, Charles A. Gersbach
Abstract
myofibers compared with exogenous PAX7 overexpression. RNA-sequencing analysis also revealed transcriptome-wide differences between myogenic progenitors generated via CRISPR-based endogenous activation of PAX7 and exogenous PAX7 cDNA overexpression. These studies demonstrate the utility of CRISPR/Cas9-based transcriptional activators for controlling cell-fate decisions.
Topics & Concepts
BiologyProgenitor cellCRISPRCell biologyCas9Induced pluripotent stem cellCellular differentiationEndogenyTranscription factorStem cellCell fate determinationEmbryonic stem cellTranscriptomeMolecular biologyGeneticsGene expressionGeneBiochemistryCRISPR and Genetic EngineeringPluripotent Stem Cells ResearchGenetics, Aging, and Longevity in Model Organisms