Isolation and identification of secondary metabolite acetone extract Aaptos sp. and its antioxidant properties and acute toxicity
Adryan Fristiohady, Baru Sadarun, Wahyuni Wahyuni, Muhammad Hajrul Malaka, Fandi Ahmad, Fadhliyah Malik, La Ode Muhammad Julian Purnama, Idin Sahidin
Abstract
Aaptos sp. can be developed and utilized as a new antioxidant source. This study aims to investigate the antioxidant activity and its acute toxicity of acetone ASE stands for Aaptos sp. Extract and its isolates. Aaptos sp. was extracted by acetone, followed by fractionating with vacuum liquid chromatography, liquid-liquid partition, and radial chromatography. Each step was intervened with thin-layer chromatography. Isolates identified by comparing their physical properties and 1 H and 13 C NMR stands for Nuclear Magnetic Resonance spectrum with literature data. Antioxidant activity assayed qualitatively and quantitatively, and the acute toxicity assayed with brine shrimp lethality test. Isolates of ASE (44 g) obtained were AS1 (50 mg), AS2 (23 mg), AS3 (8.3 mg), and AS4 (22 mg). AS1 is identified as cholestanol. Antioxidant activity assayed qualitatively showed that ASE, AS1, AS2, and AS5 were showing as antioxidant activity, only ASE had IC 50 values 16.10 g/ml. LC 50 of ASE, AS1, AS2, and AS5 were 1,041.5 g/ml; 1,488.33 g/ml; 681,87 g/ml; and 783,21 g/ml, respectively. In conclusion, there are four isolates from the ASE although only cholestanol (AS1) successfully identified. ASE, AS1, AS2, and AS5 have antioxidant activity but only IC 50 of ASE was measured and they are regarded as safe with LC 50 > 1,000 for ASE and LC 50 > 200 for its isolates.