UPLC-PDA-ESI-QTOF-MS/MS fingerprint of purified flavonoid enriched fraction of <i>Bryophyllum pinnatum</i> ; antioxidant properties, anticholinesterase activity and in <i>silico</i> studies
Joyce Oloaigbe Ogidigo, Chioma Assumpta Anosike, Parker Elijah Joshua, Collins U. Ibeji, Daniel Emmanuel Ekpo, Bennett C. Nwanguma, O. F. C. Nwodo
Abstract
CONTEXT: (Lam.) Oken (Crassulaceae) is used traditionally to treat many ailments. OBJECTIVES: approaches. MATERIALS AND METHODS: for 4 months. Molecular docking was performed to give insight into the binding potentials of BPFRF constituents against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). RESULTS: values of 22.283 ± 0.27 µg/mL and 33.437 ± 1.46 µg/mL, respectively compared to quercetin and rivastigmine. Docking studies revealed that luteolin-7-glucoside, carlinoside and quercetin interact effectively with crucial amino acid residues of AChE and BuChE through hydrogen bonds. DISCUSSION AND CONCLUSIONS: BPFRF possesses an excellent natural source of cholinesterase inhibitor and antioxidant. The material could be further explored for the potential treatment of oxidative damage and cholinergic dysfunction in Alzheimer's disease.