Molecular typing of clinical and environmental isolates of <i>Klebsiella pneumoniae</i> producing ESBLs by PFGE.
Mohammad Esmaeilnia, Mahmood Saffari, Somaye Rashki, Zeynab Marzhoseyni, Azad Khaledi, Gholam Abbas Moosavi, Fatemeh Atoof, Behrang Alani
Abstract
Objectives: isolates recovered from Beheshti Hospital, Kashan, Iran. Materials and Methods: isolates producing ESBLs have been collected from the samples obtained from Shahid Beheshti hospital, Kashan, Iran. Antimicrobial susceptibility was determined using the Kirby Bauer disk diffusion method. The presence of ESBLs was evaluated using CLSI for ESBL screening by the double-disk diffusion method. Molecular typing was conducted by pulsed-field gel electrophoresis (PFGE). In total, 89 K. pneumoniae isolates were recovered, of which 47.1% were ESBL producers. Results: =0.2). Conclusion: isolates, indicating a similar genotype and likely a common origin for ESBL producer isolates found in different hospital wards. Therefore, hospitals need to implement an effective infection control system to decrease the spreading of ESBL strains within the hospitals and subsequently the transmission of the infection to patients.