Characterization of TelE, a T7SS LXG Effector Exhibiting a Conserved C-Terminal Glycine Zipper Motif Required for Toxicity
Wooi Keong Teh, Yichen Ding, Francesca Gubellini, Alain Filloux, Claire Poyart, Michael Givskov, Shaynoor Dramsi
Abstract
strain UCN34 secretes six T7SSb effectors. One of the six effectors named TelE displayed a strong toxicity when overexpressed in Escherichia coli. Our results indicate that TelE is probably a pore-forming toxin whose activity can be antagonized by a specific immunity protein named TipE. Overall, we report a unique toxin-immunity protein pair and our data expand the range of effectors secreted through T7SSb.
Topics & Concepts
EffectorBiologySecretionGeneGeneticsMutantLocus (genetics)Cell biologyBiochemistryGenomics and Phylogenetic StudiesStreptococcal Infections and TreatmentsBacterial Identification and Susceptibility Testing