Sensitive and rapid determination of heat shock protein 70 using lateral flow immunostrips and upconversion nanoparticle fluorescence probes
Nengying Wu, Yuxi Wei, Lanlan Pan, Xiaolin Yang, Honglan Qi, Qiang Gao, Chengxiao Zhang, Chenzhong Li
Abstract
, small sample volume (120 μL), short assay time (15 min) and good reproducibility. The fluorescence method was successfully applied in the determination of Hsp70 in serum samples with good recovery. By combining the accessibility of the lateral flow immunostrips and upconversion nanoparticles, the fluorescence method can serve as a point-of-care testing method for protein assays with high sensitivity and fast detection.
Topics & Concepts
Detection limitFluorescenceMaterials scienceNanoparticleImmunoassayPhoton upconversionAnalytical Chemistry (journal)Hsp70ChemistryChromatographyHeat shock proteinNanotechnologyAntibodyLuminescenceBiochemistryOptoelectronicsOpticsPhysicsImmunologyGeneBiologyMass Spectrometry Techniques and ApplicationsAir Quality and Health Impactsthermodynamics and calorimetric analyses