Novel Fluorescence-Based High-Throughput FLIPR Assay Utilizing Membrane-Tethered Genetic Calcium Sensors to Identify T-Type Calcium Channel Modulators
Yanling Zhang, Sean P. Moran, Andrew S. Allen, David Báez-Nieto, Qihong Xu, Lei Wang, William E. Martenis, Joshua R. Sacher, Jennifer Gale, Michel Weïwer, Florence F. Wagner, Jen Q. Pan
Abstract
channels. Lastly, we demonstrate the utility of this novel fluorescence-based assay to evaluate the activities of two distinct G-protein-coupled receptors, thus expanding the use of GCaMP6s-CAAX to a wide range of applications relevant for developing cellular assays in drug discovery.
Topics & Concepts
N-type calcium channelCalciumCalcium channelThroughputFluorescenceHigh-throughput screeningChemistryVoltage-dependent calcium channelMembraneChannel (broadcasting)BiophysicsT-type calcium channelCell biologyComputer scienceBiochemistryBiologyComputer networkTelecommunicationsPhysicsOrganic chemistryQuantum mechanicsWirelessIon channel regulation and functionNeuroscience and Neural EngineeringNeuroscience and Neuropharmacology Research