Encoding latent SuFEx reactive meta-fluorosulfate tyrosine to expand covalent bonding of proteins
Paul C. Klauser, Viktoriya Y. Berdan, Li Cao, Lei Wang
Abstract
proximity-enabled SuFEx reaction. mFSY was able to target residues that were elusive for previous unnatural amino acids, and permitted engineering of various proteins including affibody, nanobody, and Fab into covalent binders that irreversibly cross-linked EGFR and HER2. mFSY is thus valuable for developing covalent proteins for biological research, synthetic biology, and biotherapeutics.
Topics & Concepts
Covalent bondChemistryTyrosinePosttranslational modificationBiochemistryCombinatorial chemistryEnzymeOrganic chemistryMonoclonal and Polyclonal Antibodies ResearchClick Chemistry and ApplicationsRNA and protein synthesis mechanisms