Adhesion analysis via a tumor vasculature-like microfluidic device identifies CD8+ T cells with enhanced tumor homing to improve cell therapy
Camila P. Camargo, Abir K. Muhuri, Yunus Alapan, Lauren F. Sestito, Megha Khosla, Margaret P. Manspeaker, Aubrey S. Smith, Chrystal M. Paulos, Susan N. Thomas
Abstract
T cells with enhanced adhesion in flow in vitro and tumor homing in vivo improve tumor control by ACT in combination with immune checkpoint blockade. These results show that engineered microfluidic devices can model the microenvironment of the tumor vasculature to identify subsets of T cells with enhanced tumor infiltrating capabilities, a key limitation in ACT.
Topics & Concepts
Homing (biology)Tumor microenvironmentCD8Cancer researchEx vivoCell biologyT cellCytotoxic T cellAdoptive cell transferCell adhesionChemistryImmune systemCellImmunologyBiologyIn vitroTumor cellsBiochemistryEcologyCAR-T cell therapy researchImmune Cell Function and InteractionImmunotherapy and Immune Responses