High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler
Taras Sych, Jan Schlegel, Hanna M. G. Barriga, Miina Ojansivu, Leo Hanke, Florian Weber, Rakibe Beklem Bostancıoğlu, Kariem Ezzat, Herbert Stangl, Birgit Plochberger, Jurga Laurencikiene, Samir EL Andaloussi, Daniel Fürth, Molly M. Stevens, Erdinç Sezgin
Abstract
We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5-200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.
Topics & Concepts
Particle sizeLiposomeNanoparticleParticle (ecology)NanotechnologyNano-RNAMaterials scienceMicrovesiclesBiological systemChemistryChromatographyBiochemistryBiologymicroRNAComposite materialPhysical chemistryGeneEcologyRNA Interference and Gene DeliveryLipid Membrane Structure and BehaviorExtracellular vesicles in disease