High Selectivity Cofactor NADH Regeneration Organic Iridium Complexes Used for High-Efficiency Chem-Enzyme Cascade Catalytic Hydrogen Transfer
Lijun Zhao, Caimei Zhang, Shixin Zhang, Xiaoyi Lv, Jia‐Yang Chen, Xun Sun, Huijuan Su, Toru Murayama, Caixia Qi
Abstract
Our research demonstrated that novel pentamethylcyclopentadienyl (Cp*) iridium pyridine sulfonamide complex PySO 2 NPh-Ir ( 7 ) could highly specifically catalyze nicotinamide adenine dinucleotide (NAD + ) into the corresponding reducing cofactor NADH in cell growth media containing various biomolecules. The structures and catalytic mechanism of 7 were studied by single-crystal X-ray, NMR, electrochemical, and kinetic methods, and the formation of iridium hydride species Ir–H was confirmed to be the plausible hydride-transfer intermediate of 7 . Moreover, benefiting from its high hydrogen-transfer activity and selectivity for NADH regeneration, 7 was used as an optimal metal catalyst to establish a chem-enzyme cascade catalytic hydrogen-transfer system, which realized the high-efficiency preparation of l -glutamic acid by combining with l -glutamate dehydrogenase (GLDH).