A Highly Compatible Phototrophic Community for Carbon‐Negative Biosynthesis
Chaofeng Li, Ruoyu Wang, Jiawei Wang, Liangxu Liu, Hengrun Li, Haotian Zheng, Jun Ni
Abstract
Abstract CO 2 sequestration engineering is promising for carbon‐negative biosynthesis, and artificial communities can solve more complex problems than monocultures. However, obtaining an ideal photosynthetic community is still a great challenge. Herein, we describe the development of a highly compatible photosynthetic community (HCPC) by integrating a sucrose‐producing CO 2 sequestration module and a super‐coupled module. The cyanobacteria CO 2 sequestration module was obtained using stepwise metabolic engineering and then coupled with the efficient sucrose utilization module Vibrio natriegens . Integrated omics analysis indicated that enhanced photosynthetic electron transport and extracellular vesicles promote intercellular communication. Additionally, the HCPC was used to channel CO 2 into valuable chemicals, enabling the overall release of −22.27 to −606.59 kgCO 2 e kg −1 in the end products. This novel light‐driven community could facilitate circular economic implementation in the future.