Cell-free biocatalytic syntheses of <scp>l</scp>-pipecolic acid: a dual strategy approach and process intensification in flow
David Roura Padrosa, Ana I. Benítez‐Mateos, Liam Calvey, Francesca Paradisi
Abstract
As an alternative to the traditional chemical synthesis or <italic>in vivo</italic> production of <sc>l</sc>-pipecolic acid, we have developed two <italic>ex vivo</italic> strategies using purified and immobilised enzymes for the production of this key building block.
Topics & Concepts
Pipecolic acidChemistryEx vivoBiocatalysisEnzymeCombinatorial chemistryBiochemistryCatalysisAmino acidIn vitroReaction mechanismEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and BioproductionPancreatic function and diabetes