A Novel NIR Fluorescent Probe for Highly Selective Detection of Nitroreductase and Hypoxic-Tumor-Cell Imaging
Feng Liu, Hong Zhang, Kun Li, Yongmei Xie, Zhihui Li
Abstract
Nitroreductase as a potential biomarker for aggressive tumors has received extensive attention. In this work, a novel NIR fluorescent probe for nitroreductase detection was synthesized. The probe Py-SiRh-NTR displayed excellent sensitivity and selectivity. Most importantly, the confocal fluorescence imaging demonstrated that HepG-2 cells treated with Py-SiRh-NTR under hypoxic conditions showed obvious enhanced fluorescence, which means that the NTR was overexpressed under hypoxic conditions. Moreover, the probe showed great promise that could help us to study related anticancer mechanisms research.
Topics & Concepts
NitroreductaseFluorescenceFluorescence-lifetime imaging microscopyChemistryConfocalBiophysicsSelectivityBiochemistryEnzymeBiologyOpticsCatalysisPhysicsNanoplatforms for cancer theranosticsAdvanced Nanomaterials in CatalysisAdvanced biosensing and bioanalysis techniques