Affimer Tagged Cubosomes: Targeting of Carcinoembryonic Antigen Expressing Colorectal Cancer Cells Using <i>In Vitro</i> and <i>In Vivo</i> Models
A. Pramanik, Zexi Xu, Shazana Hilda Shamsuddin, Yazan S. Khaled, Nicola Ingram, Thomas Maisey, Darren C. Tomlinson, P. Louise Coletta, David Jayne, Thomas A. Hughes, Arwen I. I. Tyler, Paul A. Millner
Abstract
in colorectal cancer mouse xenografts, while exhibiting low nonspecific absorption and toxicity in other vital organs. Cancerous spheroids had maximum cell death compared to noncancerous cells upon targeted delivery. Xenografts subjected to targeted drug-loaded cubosomes showed a 5-7-fold higher drug accumulation in the tumor tissue compared to the liver, kidneys, and other vital organs, a significant decrease in tumor growth, and an increased survival rate compared to the nontargeted group. This work encompasses the first thorough preclinical investigation of Affimer targeted cubosomes as a cancer therapeutic.