Comparative Bioevaluation of 99mTc Tricarbonyl and 99mTc-Sn(II) Lansoprazole as a Model for Peptic Ulcer Localization
M. H. Sanad, H. M. Eyssa, F. A. Marzook, Ayman Farag, Syed Faheem Askari Rizvi, Sudip Kumar Mandal, Soumya Stuti Patnaik, A. S. M. Fouzy, Sabry A. Bassem, Francis Verpoort
Abstract
Two 99mTc cores, 99mTc tricarbonyl and 99mTc-Sn(II), are compared in labeling of Lansoprazole (Lans). The optimum conditions of Lansoprazole labeling with 99mTc in the presence of SnCl2·2H2O as a reducing agent are as follows: pH 8, 100 μg of SnCl2·2H2O, room temperature, 2 mg of lansoprazole, and 30 min; the maximum radiochemical yield was 98 ± 0.22%. The obtained 99mTc Lansoprazole complex was stable in serum for up to 16 h. The optimum conditions of Lansoprazole binding with the 99mTc tricarbonyl ([99mTc(CO)3(H2O)3]+) core are as follows: pH 8, 2 mg of Lansoprazole, 100°С, 30 min. The complex was stable in serum for up to 12 h. Biodistribution study of the first core on two different groups of mice (control group and ulcer-bearing group) showed comparatively high uptake in the ulcer group of mice, 77.8 ± 1.1% ID/g at 30 min post-injection (higher than for the control group); at 24 h, the uptake decreased to 17.9% ID/g. Similar results were obtained with 99mTc tricarbonyl Lansoprazole: 78.5% ID/g at 30 min post-injection and 24.6% at 24 h. Thus, both complexes are suitable as radiotracers for stomach ulcer imaging.