Next-generation pathogen diagnosis with CRISPR/Cas-based detection methods
Xinjie Wang, Xiaoyun Shang, Xingxu Huang
Abstract
Ideal methods for detecting pathogens should be sensitive, specific, rapid, cost-effective and instrument-free. Conventional nucleic acid pathogen detection strategies, mostly PCR-based techniques, have various limitations, such as expensive equipment, reagents and skilled performance. Recently, CRISPR/Cas-based methods have burst onto the scene, with the potential to power the pathogen detection field. Here we introduce these unique methods and discuss its hurdles and promises.
Topics & Concepts
CRISPRComputer scienceComputational biologyPathogenNucleic acid detectionBiologyNucleic acidMicrobiologyGeneticsGeneCRISPR and Genetic EngineeringMosquito-borne diseases and controlBiosensors and Analytical Detection