Quantitative analysis of heparan sulfate using isotopically labeled calibrants
Zhangjie Wang, Katelyn Arnold, Yongmei Xu, Vijayakanth Pagadala, Guowei Su, Hannah Myatt, Robert J. Linhardt, Jian Liu
Abstract
Abstract Heparan sulfate is a sulfated polysaccharide that displays essential physiological functions. Here, we report a LC-MS/MS-based method for quantitatively determining the individual disaccharide composition and total amount of heparan sulfate. Using eight 13 C-labeled disaccharide calibrants and one 13 C-labeled polysaccharide calibrant, we complete the analysis in one-pot process. The method is both sensitive and has the throughput to analyze heparan sulfate from mouse tissues and plasma.
Topics & Concepts
Heparan sulfateDisaccharideSulfationChemistrySulfatePolysaccharideChromatographyGlycosaminoglycanBiochemistryOrganic chemistryProteoglycans and glycosaminoglycans researchGlycosylation and Glycoproteins ResearchPlatelet Disorders and Treatments