Characterization of Simultaneous Aerobic Denitrification and Dephosphorization Strain<i>Cupriavidus</i>sp. H29 and Its Application on Cadmium-Removing
Junfeng Su, Hao Zhang, Lei Xue, Yuanming Zhang
Abstract
A cadmium tolerant strain Cupriavidus sp. H29 could be applied on simultaneous removal of nitrate, phosphorus and cadmium. Response surface methodology (RSM) experiments showed that optimal removal ratios of nitrate, phosphate and Cd(II), which reached 98.89%, 75.23% and 85.01%, occurred at Cd(II) initial concentration of 30.00 mg/L, nitrate initial concentration of 55.20 mg/L, phosphate initial concentration of 50.00 mg/L, initial pH of 7.0 and C/N ratio of 6.0. Studies on gaseous product, precipitations and extracellular polymeric substances (EPS) indicated that the removal of Cd(II) occurred in the extracellular place. Through the coordinated complexation of EPS, strain H29 can achieve the bio-induced phosphate-cadmium removal. Moreover, studies on heated cells, resting cells, permeable cells, cells membrane and cytoplasm demonstrated that the removal of Cd(II) mainly taken place on the cells membrane. This study provided the theoretical basis for the subsequent research of synchronous removal of heavy metals and other pollutants.