Chemical modifications of proteins and their applications in metalloenzyme studies
Nathchar Naowarojna, Ronghai Cheng, Juan Lopez, Christina Wong, Lu Qiao, Pinghua Liu
Abstract
Protein chemical modifications are important tools for elucidating chemical and biological functions of proteins. Several strategies have been developed to implement these modifications, including enzymatic tailoring reactions, unnatural amino acid incorporation using the expanded genetic codes, and recognition-driven transformations. These technologies have been applied in metalloenzyme studies, specifically in dissecting their mechanisms, improving their enzymatic activities, and creating artificial enzymes with non-natural activities. Herein, we summarize some of the recent efforts in these areas with an emphasis on a few metalloenzyme case studies.
Topics & Concepts
ChemistryEnzymeBiochemical engineeringBiochemistryComputational biologyAmino acid residueBiologyEngineeringPeptide sequenceGeneAdvanced biosensing and bioanalysis techniquesPeptidase Inhibition and AnalysisClick Chemistry and Applications