The influence of coating with aminopropyl triethoxysilane and CuO/Cu<sub>2</sub>O nanoparticles on antimicrobial activity of cotton fabrics under dark conditions
Darka Marković, Jelena Vasiljević, Jelena Ašanin, Tatjana Ilic‐Tomic, Brigita Tomšič, Bojan Jokić, Miodrag Mitrić, Barbara Simončić, Dušan Mišić, Maja Radetić
Abstract
Abstract A novel impregnation process for the fabrication of cotton nanocomposite with strong antimicrobial activity against antibiotics‐resistant bacteria and yeast was developed. The impregnation process includes the sol–gel treatment of fabric with (3‐aminopropyl)triethoxysilane in the first step, and synthesis of the CuO/Cu 2 O nanoparticles (NPs) on the fabric surface in the second step. The in situ synthesis of the CuO/Cu 2 O NPs was based on the adsorption of Cu 2+ ‐ions by the introduced amino groups of the sol–gel coating. The adsorbed Cu 2+ ‐ions are subsequently reduced in the alkaline solution of NaBH 4 . X‐ray diffraction measurements confirmed the formation of CuO/Cu 2 O NPs. Scanning electron microscopy and atomic absorption spectrometry analyses indicate that the particle size, agglomeration, and amounts of synthesized NPs were highly affected by the initial concentration of CuSO 4 solution. The toxicity of nanocomposites to human keratinocytes (HaCaT) and antimicrobial activity against Gram‐negative Escherichia coli ATCC 25922, E. coli ATCC BAA 2469, and Klebsiella pneumoniae ATCC BAA 2146, and Gram‐positive bacteria Staphylococcus aureus ATCC 25923, S. aureus ATCC 43300 and yeast Candida albicans ATCC 24433 strongly depended on the copper content. In addition to excellent antimicrobial activity, controlled release of Cu 2+ ‐ions from the fabrics into physiological saline solution was obtained.