Distribution and Molecular Characterization of Functional Class 2 Integrons in Clinical <i>Proteus mirabilis</i> Isolates.
Wenjun Lu, Quedan Qiu, Keda Chen, Rongqing Zhao, Qingcao Li, Qiaoping Wu
Abstract
BACKGROUND: ) isolates from patients, and molecular characterization of functional class 2 integrons was further analyzed. METHODS: isolates. The variable regions of the integrons were determined by restriction analysis and sequencing. Internal stop codons mutations in class 2 integrons and their common promoters were also determined by sequencing. Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) was used to analyze the phylogenetic relations of class 2 integron-positive isolates. RESULTS: and Pc promoters were detected in these three functional class 2 integron isolates, as was found in other class 2 integron isolates. However, these three strains did not totally show identical homology and drug sensitivity. CONCLUSION: Although functional class 2 integrons have low distribution and relatively conserved molecular characteristics, they can still form clinical dissemination and drug resistance expression.