circ-LDLRAD3 Knockdown Reduces Cisplatin Chemoresistance and Inhibits the Development of Gastric Cancer with Cisplatin Resistance through miR-588 Enrichment-Mediated SOX5 Inhibition
Qianping Liang, Feifei Chu, Lei Zhang, Yuanyuan Jiang, Lu Li, Huili Wu
Abstract
Background/Aims: Chemoresistance is a common event after cancer chemotherapy, which is associated with the deregulation of circular RNAs (circRNAs). The objective of this study was to clarify the role of circ-LDLRAD3 in cisplatin (DDP)-resistant gastric cancer (GC). Methods: . Exosomes were isolated by differential centrifugation and identified by transmission electron microscopy and the expression of exosome-related proteins. Results: . circ-LDLRAD3 was overexpressed in exosomes isolated from DDP-resistant GC cells. Conclusions: circ-LDLRAD3 knockdown reduced DDP resistance and blocked the malignant development of DDP-resistant GC by modulating the miR-588/SOX5 pathway.
Topics & Concepts
Gene knockdownCisplatinCell growthApoptosisMolecular biologyCancer researchFlow cytometryGrowth inhibitionViability assayCancer cellmicroRNABiologyChemistryCancerGeneChemotherapyBiochemistryGeneticsCircular RNAs in diseasesCancer-related molecular mechanisms researchExtracellular vesicles in disease