Rapid SARS-CoV-2 Variants Enzymatic Detection (SAVED) by CRISPR-Cas12a
Jun Yang, Nilakshi Barua, Md. Nannur Rahman, Carmen Li, Norman Lo, Kai-Yan Yeong, Tsz Fung Tsang, Xiao Yang, Yuk‐Yam Cheung, Alan K. L. Tsang, Rickjason C. W. Chan, Eddie Chi‐Man Leung, Paul K.S. Chan, Margaret Ip
Abstract
etection (SAVED) method based on CRISPR-Cas12a that targets previously significant variants like Alpha, Beta, Gamma, Delta, Lambda, Mu, and Kappa as well as the VOC Omicron and its subvariants BA.1, BA.2, BA.3, BA.4, and BA.5 that are currently circulating. SAVED uses no sophisticated instruments and is reasonably priced ($3.23 per reaction). As the mutation location of these variations may reoccur in subsequent variants, we offer a system that can be applied for variant discrimination with ease and allows for adjustments to integrate newer incoming variants.