Structural elucidation and isomeric differentiation/quantitation of monophosphorylated phosphoinositides using gas-phase ion/ion reactions and dissociation kinetics
Kimberly C. Fabijanczuk, Hsi‐Chun Chao, Joshua L. Fischer, Scott A. McLuckey
Abstract
a charge inversion ion/ion reaction. Utilizing sequential ion/ion reactions and subsequent activation yields product ion information that is of limited utility for phosphorylation site localization. However, the kinetics of dissociation allowed for isomeric differentiation of the position of the phosphate group. Furthermore, employing the same kinetics method, relative quantitative information was gained for the isomeric species.
Topics & Concepts
Dissociation (chemistry)ChemistryIonKineticsDeprotonationGas phaseInorganic chemistryOrganic chemistryPhysicsQuantum mechanicsMass Spectrometry Techniques and ApplicationsProtein Kinase Regulation and GTPase SignalingLipid metabolism and biosynthesis