Ultrafast and selective labeling of endogenous proteins using affinity-based benzotriazole chemistry
Xiaoyi Xin, Yu Zhang, Massimiliano Gaetani, Susanna L. Lundström, Roman A. Zubarev, Yuan Zhou, Dale Corkery, Yao‐Wen Wu
Abstract
Affinity-based benzotriazole (BTA) probes selectively and covalently label native proteins or endogenous proteins in cells with a fast reaction rate. It is enormously useful for characterizing protein function in biological systems and for drug development.
Topics & Concepts
BenzotriazoleChemistryEndogenyUltrashort pulseCombinatorial chemistryAffinity labelingBiophysicsBiochemistryOrganic chemistryBinding siteBiologyLaserOpticsPhysicsClick Chemistry and ApplicationsChemical Synthesis and AnalysisBiotin and Related Studies