Weak promoters to drive selection marker expression: Improvement of cell line development process for therapeutic protein production in CHO-K1 cells
Lucie Grindes, Camille Florimond, Sébastien Ribault, Céline Raymond, Wilfrid Dieryck, Gilles Joucla, Cyrielle Corbin
Topics & Concepts
Chinese hamster ovary cellBiologyTransfectionPromoterMonoclonal antibodyCell cultureclone (Java method)Molecular biologyPuromycinCell biologyGene expressionAntibodyGeneGeneticsProtein biosynthesisViral Infectious Diseases and Gene Expression in InsectsVirus-based gene therapy researchProtein purification and stability