HPLC Estimation of Flavanoid (quercetin) of leaves and stem extracts of Ocimum sanctum and Tinospora cordifolia

Abstract

High performance liquid chromatography method is used for the estimation of flavanoid (quercetin) in leaves and stem extracts of Ocimum sanctum and Tinospora cordifolia. The dried leaves and stem of both plants were extracted with methanol and ethanol solvent. The concentration of quercetin of methanolic and ethanolic leaves and stem extracts of plants performed by using acetonitrile and methanol 50:50 (V/V) solutions as mobile phase and used a flow rate of 1ml per min and absorbance at 256 nm. We conclude in this study that Ocimum sanctum and Tinospora cordifolia has significant amount of flavanoid (quercetin), which indicate the high antioxidant activity of both plants and used for further study.

Topics & Concepts