Chitosan-G-Glycidyl Methacrylate/Au Nanocomposites Promote Accelerated Skin Wound Healing
Héctor A. López-Muñoz, Mauricio Lopez-Romero, Moisés Ármides Franco-Molina, Alejandro Manzano-Ramírez, Cristina Velasquillo, Beatriz Liliana España‐Sánchez, Ana Laura Martínez‐Hernández, Haydé Azeneth Vergara‐Castañeda, Astrid L. Giraldo-Betancur, Sarai E. Favela-Camacho, Rogelio Rodríguez‐Rodríguez, Juan Carlos Mixteco-Sánchez, Juan Carlos Tapia-Picazo, Diana Ginette Zárate-Triviño, E. Prokhorov, Gabriel Luna‐Bárcenas
Abstract
Herein, we report the synthesis of Au nanoparticles (AuNPs) in chitosan (CTS) solution by chemically reducing HAuCl4. CTS was further functionalized with glycidyl methacrylate (chitosan-g-glycidyl methacrylate/AuNP, CTS-g-GMA/AuNP) to improve the mechanical properties for cellular regeneration requirements of CTS-g-GMA/AuNP. Our nanocomposites promote excellent cellular viability and have a positive effect on cytokine regulation in the inflammatory and anti-inflammatory response of skin cells. After 40 days of nanocomposite exposure to a skin wound, we showed that our films have a greater skin wound healing capacity than a commercial film (TheraForm®), and the presence of the collagen allows better cosmetic ave aspects in skin regeneration in comparison with a nanocomposite with an absence of this protein. Electrical percolation phenomena in such nanocomposites were used as guiding tools for the best nanocomposite performance. Our results suggest that chitosan-based Au nanocomposites show great potential for skin wound repair.