A multi-photon fluorescent probe based on quinoline groups for the highly selective and sensitive detection of lipid droplets
Sijing Zhang, Zhenghui Yang, Minghao Li, Qiong Zhang, Xiaohe Tian, Dandan Li, Shengli Li, Jieying Wu, Yupeng Tian
Abstract
Compared to general fluorescent probes, multi-photon fluorescent probes exhibit deeper tissue penetration, lower auto-fluorescence and lower photo-toxicity in the bio-imaging field. Herein, we synthesized a series multi-photon fluorescent probe (L1-L3) based on quinolone groups. Of notably, the three-photon fluorescence of L3 significantly enhanced when L3 interacted with liposome; moreover, L3 exhibited high selectivity towards lipid droplets in living cells. Due to its large Stokes shift, high selectivity and photon-stability, L3 was successfully used in lipid droplet imaging via multi-photon fluorescence bio-imaging.
Topics & Concepts
FluorescenceQuinolineChemistryLiposomeTwo-photon excitation microscopyStokes shiftFluorescence-lifetime imaging microscopySelectivityPhotochemistryFluorescence in the life sciencesBiophysicsLaser-induced fluorescenceOpticsBiochemistryOrganic chemistryBiologyCatalysisPhysicsLipid metabolism and biosynthesisPhotosynthetic Processes and MechanismsPeroxisome Proliferator-Activated Receptors