Molecular Prosthetics for Long-Term Functional Imaging with Fluorescent Reporters
Vincent Grenier, Kayli N. Martinez, Brittany Benlian, Derek García-Almedina, Benjamin K. Raliski, Steven Boggess, Johnathan C. Maza, Samantha Yang, Anneliese M. M. Gest, Evan W. Miller
Abstract
Voltage-sensitive fluorescent reporters can reveal fast changes in the membrane potential in neurons and cardiomyocytes. However, in many cases, illumination in the presence of the fluorescent reporters results in disruptions to the action potential shape that limits the length of recording sessions. We show here that a molecular prosthetic approach, previously limited to fluorophores, rather than indicators, can be used to substantially prolong imaging in neurons and cardiomyocytes.
Topics & Concepts
FluorescenceFluorescence-lifetime imaging microscopyMolecular imagingFluorescent proteinBiophysicsNanotechnologyChemistryNeuroscienceMaterials scienceBiologyGreen fluorescent proteinOpticsPhysicsBiochemistryGeneIn vivoBiotechnologyNeuroscience and Neural EngineeringAdvanced Fluorescence Microscopy TechniquesForce Microscopy Techniques and Applications