Single-molecule microfluidic assay for prostate-specific antigen based on magnetic beads and upconversion nanoparticles
Dorota Sklenárová, Antonín Hlaváček, Jana Křivánková, Julian C. Brandmeier, Julie Weisová, Michal Říháček, Hans H. Gorris, Petr Skládal, Zdeněk Farka
Abstract
(36 fM) in 50% fetal bovine serum, which is an 11-fold improvement over the respective analog MB-based ULISA. The microfluidic technique conferred several other advantages, such as easy implementation and the potential for achieving high-throughput analysis. Finally, it was proven that the microfluidic setup is suitable for clinical sample analysis, showing a good correlation with a reference electrochemiluminescence assay (recovery rates between 97% and 105%).
Topics & Concepts
MicrofluidicsPhoton upconversionProstate-specific antigenMagnetic nanoparticlesAntigenNanotechnologyNanoparticleMaterials scienceChemistryMolecular biologyProstateBiologyOptoelectronicsCancerImmunologyGeneticsLuminescenceInnovative Microfluidic and Catalytic Techniques InnovationBiosensors and Analytical Detection