Selectivity in Enzymatic Phosphorus Recycling from Biopolymers: Isotope Effect, Reactivity Kinetics, and Molecular Docking with Fungal and Plant Phosphatases
Mina Solhtalab, Spencer R. Moller, April Z. Gu, Deb P. Jaisi, Ludmilla Aristilde
Abstract
P nuclear magnetic resonance, respectively. Molecular modeling with eight plant and fungal AP structures predicted substrate binding interactions consistent with the relative reactivity kinetics. Our findings implied a hierarchy in enzymatic P recycling from P-polymers by phosphatases from different biological origins, thereby influencing the relatively longer residence time of RNA versus polyP in environmental matrices. This research further sheds light on engineering strategies to enhance enzymatic recycling of biopolymer-derived P, in addition to advancing environmental predictions of this P recycling by plants and microorganisms.
Topics & Concepts
ChemistryPhytaseBiochemistryPhosphataseDephosphorylationEnzymeRNAKineticsEnzyme kineticsActive sitePhysicsQuantum mechanicsGenePhytase and its ApplicationsPhosphorus and nutrient managementOral and gingival health research