Simple and Fast SEC-Based Protocol to Isolate Human Plasma-Derived Extracellular Vesicles for Transcriptional Research
Laetitia S. Gaspar, Magda M. Santana, Carina Henriques, Maria M. Pinto, Teresa Ribeiro‐Rodrigues, Henrique Girão, Rui Jorge Nobre, Luís Pereira de Almeida
Abstract
particles/μg of protein, determined by Micro Bicinchoninic Acid (BCA) Protein Assay, and 3.09 ± 0.7 ng RNA, assessed by fluorescence-based RNA-quantitation, from only 900 μL of plasma. The protocol is fast and easy to implement and has potential for application in biomarkers research, therapeutic strategies development, and clinical practice.
Topics & Concepts
Extracellular vesiclesSimple (philosophy)VesicleCell biologyProtocol (science)ExtracellularHuman plasmaChemistryBiologyComputer scienceBiochemistryChromatographyMedicineMembranePathologyPhilosophyAlternative medicineEpistemologyExtracellular vesicles in diseaseRNA Interference and Gene DeliveryMicroRNA in disease regulation