Intracellular Group A <i>Streptococcus</i> Induces Golgi Fragmentation To Impair Host Defenses through Streptolysin O and NAD-Glycohydrolase
Takashi Nozawa, Junpei Iibushi, Hirotaka Toh, Atsuko Minowa‐Nozawa, Kazunori Murase, Chihiro Aikawa, Ichirô Nakagawa
Abstract
(GAS), streptolysin O (SLO) and NAD-glycohydrolase (Nga), are linked to enhanced pathogenicity of the prevalent GAS strains. Recent advances show that SLO and Nga are important for intracellular survival of GAS in epithelial cells and macrophages. Here, we found that invading GAS disrupts the Golgi complex in host cells through SLO and Nga. We show that GAS-induced Golgi fragmentation requires bacterial invasion into host cells, SLO pore formation activity, and Nga NADase activity. GAS-induced Golgi fragmentation results in the impairment of the epithelial barrier and chemokine secretion in macrophages. This immune inhibition property of SLO and Nga by intracellular GAS indicates that the invasion of GAS is associated with virulence exerted by SLO and Nga.