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Luciferase- and HaloTag-based reporter assays to measure small-molecule-induced degradation pathway in living cells

Martin P. Schwalm, Krishna Saxena, Susanne Müller, Stefan Knapp

2024Nature Protocols32 citationsDOI

Topics & Concepts

Small moleculeComputational biologyCRISPRHEK 293 cellsHigh-throughput screeningSynthetic biologyProteomicsCell biologyProtein degradationTernary complexCell cultureBiologyChemistryMolecular biologyBiochemistryGeneticsGeneEnzymeProtein Degradation and InhibitorsCAR-T cell therapy researchUbiquitin and proteasome pathways
Luciferase- and HaloTag-based reporter assays to measure small-molecule-induced degradation pathway in living cells | Litcius