Identification and assessment of cardiolipin interactions with <i>E. coli</i> inner membrane proteins
Robin A. Corey, Wanling Song, Anna L. Duncan, T. Bertie Ansell, Mark S.P. Sansom, Phillip J. Stansfeld
Abstract
inner membrane proteins. Our data reveal an asymmetry between the membrane leaflets, with increased anionic lipid binding to the inner leaflet regions of the proteins, particularly for cardiolipin. From our simulations, we identify >700 independent cardiolipin binding sites, allowing us to identify the molecular basis of a prototypical cardiolipin binding site, which we validate against structures of bacterial proteins bound to cardiolipin. This allows us to construct a set of metrics for defining a high-affinity cardiolipin binding site on bacterial membrane proteins, paving the way for a heuristic approach to defining other protein-lipid interactions.