Enhanced cytotoxic effect of doxorubicin conjugated gold nanoparticles on breast cancer model
Amna H. Faid, Samia A. Shouman, Yehia Badr, Marwa Sharaky
Abstract
BACKGROUND: The difficulty of achieving targeted drug delivery following administration of presently marketed anticancer therapeutics is still a concern. Metallic nanoparticles (NPs) appear to be promising in this regard. The present study focused on the use of gold nanoparticles (AuNPs) as a drug carrier for anticancer Doxorubicin (DOX) forming DOX-AuNPs nanocomposite. The anticancer effect of the prepared nanocomposite was evaluated using SRP essay on breast cancer cell line (MCF7) for different incubation times (24 h,48, and72hr). The prepared DOX-AuNPs nanocomposite was investigated by UV-visible spectroscopy, TEM, fluorescence spectroscopy, and FTIR spectroscopy. RESULTS: Our results showed that the prepared AuNPs and DOX-AuNPs nanocomposite have spherical and small size10 ± 2 nm and 12 ± 2 nm respectively. The potential cytotoxicity of the DOX-AuNPs nanocomposite on the MCF7 cell line was significantly increased compared to free DOX. The 20 µM DOX- AuNPs nanocomposite produced a similar decrease in cell survival as 80 µM free DOX. CONCLUSION: Future work is in progress to investigate the positive effects of the prepared nanocomposite for chemo-photothermal combination treatment.