Detection of Candida albicans Using a Manufactured Electrochemical Sensor
Prakhar Dutta, Yi-Jung Lu, Han‐Yun Hsieh, Tyng-Yuh Lee, Yi‐Tzu Lee, Chao‐Min Cheng, Yu‐Jui Fan
Abstract
Candida albicans is a commensal fungus that is responsible for a lot of nosocomial infections in immunocompromised people. Cell culture is currently the predominant method for diagnosing candidiasis, but it is time consuming. In this study, we developed a rapid screen procedure by devising a method for detecting C. albicans with the use of electrochemical sensors. Through this experiment, we propose a method for the detection of C. albicans in the system through the use of personal glucose meters. The hemicellulase was used to break down the cell wall of C. albicans to glucose and oligo, which can be detected by a glucose meter. The spiked samples were prepared suspending C. albicans in urine and serum, demonstrating the feasibility of the developed method in a real situation.