Thin Layer Chromatography (TLC) VS. Paper Chromatography: A Review
Shashank Tiwari, Shreya Talreja
Abstract
In the field of analytical chemistry and various pharmaceutical fields the separation techniques are widely used to study specific compounds from a mixture or a complex material. One such separation technique is called Chromatography. It takes place on the basis of the relative volume of each solute present in the moving fluid stream. The discovery of chromatography was done by Mikhail S. Tsvet, who was a Russian botanist in the year 1901. The separation is done through stationary phase and a mobile phase. Chromatography is used for separation, analysis, and purification of various components which can include food, pesticides, pharmaceuticals, tissue extracts etc. In this paper 2 major types of chromatography are discussed; these include Paper Chromatography and Thin Layer Chromatography. The principles of both the types will be discussed and the contrast will be established between the two in a tabular format.