Generation of single-cell and single-nuclei suspensions from embryonic and adult mouse brains

Abstract

Efficient protocols to generate single-cell and single-nuclei suspensions are critical for the burgeoning field of single-cell/single-nuclei sequencing. Here we describe procedures to generate single-cell and single-nuclei suspensions from embryonic and adult mouse brains. This protocol can be modified for any brain region and/or neural cell type. For complete details on the use and execution of this protocol, please refer to Lee et al. (2022), 1 Rhodes et al. (2022), 2 Mahadevan et al. (2021), 3 Ekins et al. (2020), 4 and Wester et al. (2019). 5

Topics & Concepts