Protocol to establish cancer-associated fibroblasts from surgically resected tissues and generate senescent fibroblasts
Tadahito Yasuda, Mayu Koiwa, Atsuko Yonemura, Takahiko Akiyama, Hideo Baba, Takatsugu Ishimoto
Abstract
Accumulating evidence suggests that the malignant behavior of cancer is influenced by stromal activity in the tumor microenvironment. Cancer-associated fibroblasts (CAFs), which are the main component of the cancerous stroma, play an important role in cancer development. Here, we describe a protocol to establish CAFs from surgically resected tissues. CAFs could be a vital tool for understanding the microenvironment and its impact on tumor progression and metastasis. Moreover, we generated inflammation-induced senescent fibroblasts that more closely mimic the tumor microenvironment. For complete details on the use and execution of this protocol, please refer to Yasuda et al. (2021).